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anti na  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti na
    Anti Na, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+na+k+atpase/Na%2CK-ATPase+alpha1+Rabbit+mAb/pmc12996945-101-42-46
    Average 95 stars, based on 85 article reviews
    anti na - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: A Novel Role of PP2A Methylation in the Regulation of Tight Junction Assembly and Integrity
    Article Snippet: Antibodies used in this study included: Mouse (clone 16B12, Covance) and rabbit (clone C29F4, Cell Signaling Technology) anti-HA; mouse anti-Myc (clone 9B11, Cell Signaling Technology); mouse anti-methyl PP2Ac (clone 2A10, Merck Millipore); mouse anti-demethyl-PP2Ac (clone 1D6; Merck Millipore); mouse anti-PP2Ac (#610556, BD Transduction); mouse anti-LCMT1 clone 4A4 (Merck Millipore); mouse anti-PME-1 ( ); rabbit ( ) and mouse (clone 2G9, Merck Millipore) anti-Bα; rabbit anti-Na + /K + ATPase (#3010, Cell Signaling Technology); mouse anti-occludin (clone OC-3F10; Thermo Fisher Scientific); mouse anti-ZO-1 (clone ZO1-1A12, Thermo Fisher Scientific); rabbit anti-Par3 (#07-330, Merck-Millipore); rabbit anti-aPKCζ (sc-216, Santa Cruz Biotechnology); mouse (clone C4, Merck Millipore) and rabbit (#AAN01, Cytoskeleton Inc.) anti-actin; mouse anti-α-tubulin (clone DM1A; Sigma).

    Article Title: Enhanced PI3K/Akt Signaling in Response to p85α Loss is Regulated by Endosomal PI3Kα and PI3P
    Article Snippet: Rabbit anti-p110α (#4249, Cell Signaling), rabbit anti-p110α (#ab40776, Abcam), rabbit anti-p110β (#3011, Cell Signaling), rabbit anti-p85α (#ab191606, Abcam), mouse anti-p85β (#MAB6777, R and D), mouse anti-PI3,4,5P 3 antibody (Z-P345, Echelon Biosciences), rabbit anti-Akt (#ab126811, Abcam), rabbit anti-Akt-HRP (#4298, Cell Signaling), rabbit anti-phospho-Akt T308 (#2965, Cell Signaling), rabbit anti-phospho-Akt S473 (#4060, Cell Signaling), rabbit anti-pAkt (OMA1-03061, Life Technologies), rabbit anti-phospho Akt (#OMA1-03061, Invitrogen), mouse anti-phospho Akt (#200-301-268S, Rockland), mouse anti-MAP4 (#sc-390286, Santa Cruz), rabbit anti-MAP4 (#A301-488A, Bethyl), mouse anti-MAP4 agarose beads (#sc-390286 AC, Santa Cruz), normal mouse IgG beads (sc-2343, Santa Cruz), rabbit anti-HA (#3724, Cell Signaling), mouse anti-HA agarose beads (#26181, ThermoFisher), anti-GST HRP conjugate (#RPN 1236V, Amersham), rabbit anti-Flag (#2368, Cell Signaling), mouse anti-Flag (#F1804, Sigma), anti-Flag M2 affinity gel (#F2426, Sigma-Aldrich), rabbit anti-GAPDH (#2118, Cell Signaling), rabbit anti-tubulin (#ab18251, Abcam), mouse anti-EEA1 (#610457, BD Biosciences), rabbit anti-EEA1 (#3288, Cell Signaling), mouse anti-TFR (#136800, Invitrogen), rabbit anti-TFR (#ab84036, Abcam), rat anti-tubulin (#ab6160, Abcam), rabbit anti-tubulin (#ab6046, Abcam), normal rabbit IgG beads (#2729, Cell Signaling), rabbit anti-EGFR beads (#5735 Cell Signaling), rabbit anti-EGFR (sc-03, Santa Cruz), mouse anti-EGFR (#sc-120, Santa Cruz), anti-beta actin (#4967), rabbit anti-Na+/K+ ATPase (#3010, Cell Signaling) and PTEN (sc-7974, Santa Cruz).

    Article Title: SELENOP modifies sporadic colorectal carcinogenesis and WNT signaling activity through LRP5/6 interactions
    Article Snippet: Full unedited blot for Figure 7A Mouse anti-FLAG (F1804, Sigma-Aldrich) Mouse anti-SELENOP (N11, VAPR) Mouse anti-FLAG (F1804, Sigma-Aldrich) Mouse anti-SELENOP (N11, VAPR) Full unedited blot for Figure 7B Mouse anti-FLAG (F1804, Sigma-Aldrich) Mouse anti-SELENOP (N11, VAPR) Full unedited blot for Figure 7C Full unedited blot for Figure 7D Rabbit anti-LRP6 (2560, Cell Signaling Technology) Rabbit anti-Na+/K+-ATPase (3010, Cell Signaling Technology) Rabbit anti-β-tubulin (2146, Cell Signaling Technology) Same blot, quantified on Odyssey Rabbit anti-LRP6 (2560, Cell Signaling Technology) Rabbit anti-Na+/K+-ATPase (3010, Cell Signaling Technology) Rabbit anti-β-tubulin (2146, Cell Signaling Technology) Rabbit anti-LRP6 (2560, Cell Signaling Technology) Rabbit anti-Na+/K+-ATPase (3010, Cell Signaling Technology) Rabbit anti-β-tubulin (2146, Cell Signaling Technology) Same blot, different contrast/brightness settings on Odyssey Full unedited blot for Figure 8B Rabbit anti-LRP6 (3395, Cell Signaling Technology) Rabbit anti-SELENOP (Proteintech Group) Rabbit anti-LRP6 (3395, Cell Signaling Technology) Rabbit anti-SELENOP (Proteintech Group) Same blot, different contrast/brightness settings on Odyssey Full unedited blot for Figure 8D Rabbit anti-LRP6 (3395, Cell Signaling Technology) Rabbit anti-V5 (13202, Cell Signaling Technology) Full unedited blot for Figure 9B Rabbit anti-LRP6 (3395, Cell Signaling Technology) Rabbit anti-V5 (13202, Cell Signaling Technology) Full unedited blot for Figure 9C Mouse anti-V5 (ab27671, abcam) Full unedited blot for Figure S1A Rabbit anti-β-tubulin (2146, Cell Signaling Technology) Rabbit anti-GPX1 (SAB2700534, Sigma-Aldrich) Same blot, different contrast/brightness settings on Odyssey Rabbit anti-β-tubulin (2146, Cell Signaling Technology) Rabbit anti-GPX1 (SAB2700534, Sigma-Aldrich) Full unedited blot for Figure S1B Rabbit anti-β-tubulin (2146, Cell Signaling Technology) Rabbit anti-GPX2 (ab137431, abcam) Same blot, different contrast/brightness settings on Odyssey Rabbit anti-β-tubulin (2146, Cell Signaling Technology) Rabbit anti-GPX2 (ab137431, abcam) Full unedited blot for Figure S9A Rabbit anti-APC (sc-7930, Santa Cruz Biotechnology) Rabbit anti-GAPDH (5174, Cell Signaling Technology) Full unedited blot for Figure S11 Mouse anti-FLAG (F1804, Sigma-Aldrich) Rabbit anti-V5 (13202, Cell Signaling Technology)

    Article Title: Restoring thalamocortical circuit dysfunction by correcting HCN channelopathy in Shank3 mutant mice
    Article Snippet: Rabbit anti Na-K-ATPase , Cell Signaling Technology , Cat. # 3010; RRID: AB_2060983.

    Western Blot:

    Article Title: Substrate-directed control of N-glycosylation in the endoplasmic reticulum calibrates signal reception at the cell-surface
    Article Snippet: ANTI-FLAG® M2 Affinity Gel was purchased from Sigma-Aldrich. .. The following primary antibodies were used: mouse anti- CCDC134 (E-5, Santa Cruz Biotechnology, 1:500); mouse anti-HSP90B1 (H-10, Santa Cruz Biotechnology, 1:2000); mouse anti-LRP5 (B-9, Santa Cruz Biotechnology, 1:500); rabbit anti-LRP6 (C5C7, Cell Signaling Technology, 1:1000 for immunoblot); mouse anti-LRP6 ectodomain (clone A59, MilliporeSigma, 2ug/sample for cell surface staining); Alexa Fluor® 488 rabbit anti-Giantin (A488-114L, Covance, 1:500 for immunofluorescence); mouse anti-ɑ-Tubulin (Clone DM1A, MilliporeSigma, 1:10000); rabbit anti-active (non-phosphorylated) β-catenin (D13A1, Cell Signaling Technology, 1:500); rabbit anti- Na/K ATPase (3010S, Cell Signaling Technology, 1:1000); rabbit anti-MESD (10958-1-AP, Proteintech, 1:1000); mouse anti-FLAG (clone M2, MilliporeSigma, 1:2000); rabbit anti-FLAG (F7425, Sigma-Aldrich, 1:2000); rabbit anti-PSAP (GTX101064, GeneTex, 1:1000); rabbit anti-STT3A (12034-1-AP, Proteintech, 1:1000); rabbit anti-STT3B (15323-1-AP, Proteintech, 1:1000); rabbit anti-OSTC (PA5-34060, Invitrogen, 1:1000); mouse anti-RPL17 (C-8, Santa Cruz Biotechnology, 1:2000); rabbit anti-Sec61b (15087-1-AP, Proteintech, 1:1000). .. Secondary antibodies conjugated to horseradish peroxidase or Alexa Fluor dyes were obtained from Jackson Laboratories and Thermo Fisher Scientific.

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: Finally, the pellet was resuspended in RIPA buffer (Beijing Solarbio Science & Technology Corp.) containing 1% Triton X-100 for further analysis. .. The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma). ..

    Staining:

    Article Title: Substrate-directed control of N-glycosylation in the endoplasmic reticulum calibrates signal reception at the cell-surface
    Article Snippet: ANTI-FLAG® M2 Affinity Gel was purchased from Sigma-Aldrich. .. The following primary antibodies were used: mouse anti- CCDC134 (E-5, Santa Cruz Biotechnology, 1:500); mouse anti-HSP90B1 (H-10, Santa Cruz Biotechnology, 1:2000); mouse anti-LRP5 (B-9, Santa Cruz Biotechnology, 1:500); rabbit anti-LRP6 (C5C7, Cell Signaling Technology, 1:1000 for immunoblot); mouse anti-LRP6 ectodomain (clone A59, MilliporeSigma, 2ug/sample for cell surface staining); Alexa Fluor® 488 rabbit anti-Giantin (A488-114L, Covance, 1:500 for immunofluorescence); mouse anti-ɑ-Tubulin (Clone DM1A, MilliporeSigma, 1:10000); rabbit anti-active (non-phosphorylated) β-catenin (D13A1, Cell Signaling Technology, 1:500); rabbit anti- Na/K ATPase (3010S, Cell Signaling Technology, 1:1000); rabbit anti-MESD (10958-1-AP, Proteintech, 1:1000); mouse anti-FLAG (clone M2, MilliporeSigma, 1:2000); rabbit anti-FLAG (F7425, Sigma-Aldrich, 1:2000); rabbit anti-PSAP (GTX101064, GeneTex, 1:1000); rabbit anti-STT3A (12034-1-AP, Proteintech, 1:1000); rabbit anti-STT3B (15323-1-AP, Proteintech, 1:1000); rabbit anti-OSTC (PA5-34060, Invitrogen, 1:1000); mouse anti-RPL17 (C-8, Santa Cruz Biotechnology, 1:2000); rabbit anti-Sec61b (15087-1-AP, Proteintech, 1:1000). .. Secondary antibodies conjugated to horseradish peroxidase or Alexa Fluor dyes were obtained from Jackson Laboratories and Thermo Fisher Scientific.

    Immunofluorescence:

    Article Title: Substrate-directed control of N-glycosylation in the endoplasmic reticulum calibrates signal reception at the cell-surface
    Article Snippet: ANTI-FLAG® M2 Affinity Gel was purchased from Sigma-Aldrich. .. The following primary antibodies were used: mouse anti- CCDC134 (E-5, Santa Cruz Biotechnology, 1:500); mouse anti-HSP90B1 (H-10, Santa Cruz Biotechnology, 1:2000); mouse anti-LRP5 (B-9, Santa Cruz Biotechnology, 1:500); rabbit anti-LRP6 (C5C7, Cell Signaling Technology, 1:1000 for immunoblot); mouse anti-LRP6 ectodomain (clone A59, MilliporeSigma, 2ug/sample for cell surface staining); Alexa Fluor® 488 rabbit anti-Giantin (A488-114L, Covance, 1:500 for immunofluorescence); mouse anti-ɑ-Tubulin (Clone DM1A, MilliporeSigma, 1:10000); rabbit anti-active (non-phosphorylated) β-catenin (D13A1, Cell Signaling Technology, 1:500); rabbit anti- Na/K ATPase (3010S, Cell Signaling Technology, 1:1000); rabbit anti-MESD (10958-1-AP, Proteintech, 1:1000); mouse anti-FLAG (clone M2, MilliporeSigma, 1:2000); rabbit anti-FLAG (F7425, Sigma-Aldrich, 1:2000); rabbit anti-PSAP (GTX101064, GeneTex, 1:1000); rabbit anti-STT3A (12034-1-AP, Proteintech, 1:1000); rabbit anti-STT3B (15323-1-AP, Proteintech, 1:1000); rabbit anti-OSTC (PA5-34060, Invitrogen, 1:1000); mouse anti-RPL17 (C-8, Santa Cruz Biotechnology, 1:2000); rabbit anti-Sec61b (15087-1-AP, Proteintech, 1:1000). .. Secondary antibodies conjugated to horseradish peroxidase or Alexa Fluor dyes were obtained from Jackson Laboratories and Thermo Fisher Scientific.

    Immunostaining:

    Article Title: STON2 variations are involved in synaptic dysfunction and schizophrenia-like behaviors by regulating Syt1 trafficking.
    Article Snippet: Finally, the pellet was resuspended in RIPA buffer (Beijing Solarbio Science & Technology Corp.) containing 1% Triton X-100 for further analysis. .. The following antibodies were used for Western blotting and immunostaining: rabbit anti-Ston2 (Sigma), rabbit anti–a-tubulin (Abcam), rabbit anti-GFP (Invitrogen), mouse anti-synaptotagmin 1 (Synaptic Systems, Cat. No. 105011), rabbit anti-SV2A (Synaptic Systems), rabbit anti-EPS15 (Sigma), mouse anti-synaptophysin (Synaptic Systems), rabbit anti-HA (Cell Signaling Technology), mouse anti-c-Myc (Santa Cruz), rabbit anti-clathrin HC (Cell Signaling Technology), anti-EPS15 (Sigma), rabbit anti-vGlut1 (Synaptic systems), mouse anti-vGat (Synaptic Systems), rabbit anti-p-AP2M1 (Cell Signaling Technology), rabbit anti-Na+-K+ ATPase (Cell Signaling Technology), anti-phosphoserine (Millipore), and mouse anti-Flag (Sigma). ..

    Incubation:

    Article Title: Biomimetic cancer cell membrane-coated liposomal nanocarriers loaded with silibinin suppress gastric cancer progression via SNHG1/miR-383-5p/HSP90AA1 axis-mediated PI3K/AKT pathway inhibition
    Article Snippet: Equal amounts of protein (20–40 μg) were separated by 10 % SDS-PAGE and transferred to PVDF membranes (Cat. No. IPVH00010, Millipore, USA). .. Membranes were blocked at ambient temperature for 2 h with 5 % non-fat milk in PBS containing 0.1 % Tween-20 (PBST), followed by overnight incubation at 4 °C with the following primary antibodies: Rabbit anti-Pan-cadherin (1:1000, ab51034, Abcam, UK), Rabbit anti-COXIV (1:1000, 4850, Cell Signaling Technology, USA), Rabbit anti-Histone H3 (1:2000, 4499, CST, USA), Rabbit anti-Na + /K + -ATPase (1:1000, 3010, CST, USA), Rabbit anti-HSP90AA1 (1:500, ab314005, Abcam, UK), Rabbit anti-E-cadherin (1:1000, 3195S, CST, USA), Rabbit anti-PI3K (1:500, ab154598, Abcam, UK), Rabbit anti-p-PI3K (1:1000, ab191606, Abcam, UK), Rabbit anti-AKT (1:500, ab8805, Abcam, UK), Rabbit anti-p-AKT (1:500, ab38449, Abcam, UK), Rabbit anti-GAPDH (1:10000, 10494-1-AP, Proteintech, China; used as loading control). .. After washing with PBST, membranes were incubated with HRP-conjugated secondary antibody (1:5000, ab205718, Abcam, UK) for 2 h at room temperature.

    Control:

    Article Title: Biomimetic cancer cell membrane-coated liposomal nanocarriers loaded with silibinin suppress gastric cancer progression via SNHG1/miR-383-5p/HSP90AA1 axis-mediated PI3K/AKT pathway inhibition
    Article Snippet: Equal amounts of protein (20–40 μg) were separated by 10 % SDS-PAGE and transferred to PVDF membranes (Cat. No. IPVH00010, Millipore, USA). .. Membranes were blocked at ambient temperature for 2 h with 5 % non-fat milk in PBS containing 0.1 % Tween-20 (PBST), followed by overnight incubation at 4 °C with the following primary antibodies: Rabbit anti-Pan-cadherin (1:1000, ab51034, Abcam, UK), Rabbit anti-COXIV (1:1000, 4850, Cell Signaling Technology, USA), Rabbit anti-Histone H3 (1:2000, 4499, CST, USA), Rabbit anti-Na + /K + -ATPase (1:1000, 3010, CST, USA), Rabbit anti-HSP90AA1 (1:500, ab314005, Abcam, UK), Rabbit anti-E-cadherin (1:1000, 3195S, CST, USA), Rabbit anti-PI3K (1:500, ab154598, Abcam, UK), Rabbit anti-p-PI3K (1:1000, ab191606, Abcam, UK), Rabbit anti-AKT (1:500, ab8805, Abcam, UK), Rabbit anti-p-AKT (1:500, ab38449, Abcam, UK), Rabbit anti-GAPDH (1:10000, 10494-1-AP, Proteintech, China; used as loading control). .. After washing with PBST, membranes were incubated with HRP-conjugated secondary antibody (1:5000, ab205718, Abcam, UK) for 2 h at room temperature.



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    Intracellular MICA/B and ULBP2 co-localize with Golgi apparatus and show different mechanism of intracellular trafficking. (A) Subcellular localization of MICA/B and ULBP2 (green) in BC cell lines, and co-localization with calnexin (endoplasmic reticulum, ER) and GM130 (Golgi) (red). Nuclei are counterstained with DAPI (blue). White arrows indicate co-localization (yellow/orange staining). (B) Calnexin or GM130 co-localization with MICA/B or ULBP2 was quantified by ImageJ plug-in JACOP. Experiment was performed twice and analyzed in at least 6 fields for each condition. Statistical analysis was performed using Kruskall-Wallis for non-parametric distribution with Dunn’s multiple comparisons within organelles: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (C) Western blotting analysis of NKG2DLs and GM130 in subcellular fractions: cytosol (C), membrane (M), nucleus (N) and cytoskeleton (CS) <t>extracts.</t> <t>TATA-binding</t> protein (TBP), β-Actin and Na + /K + <t>-ATPase</t> were used as housekeeping for each fraction.
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    Image Search Results


    Intracellular MICA/B and ULBP2 co-localize with Golgi apparatus and show different mechanism of intracellular trafficking. (A) Subcellular localization of MICA/B and ULBP2 (green) in BC cell lines, and co-localization with calnexin (endoplasmic reticulum, ER) and GM130 (Golgi) (red). Nuclei are counterstained with DAPI (blue). White arrows indicate co-localization (yellow/orange staining). (B) Calnexin or GM130 co-localization with MICA/B or ULBP2 was quantified by ImageJ plug-in JACOP. Experiment was performed twice and analyzed in at least 6 fields for each condition. Statistical analysis was performed using Kruskall-Wallis for non-parametric distribution with Dunn’s multiple comparisons within organelles: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (C) Western blotting analysis of NKG2DLs and GM130 in subcellular fractions: cytosol (C), membrane (M), nucleus (N) and cytoskeleton (CS) extracts. TATA-binding protein (TBP), β-Actin and Na + /K + -ATPase were used as housekeeping for each fraction.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Breast cancer bone metastasis and bone metastatic cells retain NKG2DLs intracellularly: could this be a strategy to evade immune recognition?

    doi: 10.3389/fcell.2026.1717607

    Figure Lengend Snippet: Intracellular MICA/B and ULBP2 co-localize with Golgi apparatus and show different mechanism of intracellular trafficking. (A) Subcellular localization of MICA/B and ULBP2 (green) in BC cell lines, and co-localization with calnexin (endoplasmic reticulum, ER) and GM130 (Golgi) (red). Nuclei are counterstained with DAPI (blue). White arrows indicate co-localization (yellow/orange staining). (B) Calnexin or GM130 co-localization with MICA/B or ULBP2 was quantified by ImageJ plug-in JACOP. Experiment was performed twice and analyzed in at least 6 fields for each condition. Statistical analysis was performed using Kruskall-Wallis for non-parametric distribution with Dunn’s multiple comparisons within organelles: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (C) Western blotting analysis of NKG2DLs and GM130 in subcellular fractions: cytosol (C), membrane (M), nucleus (N) and cytoskeleton (CS) extracts. TATA-binding protein (TBP), β-Actin and Na + /K + -ATPase were used as housekeeping for each fraction.

    Article Snippet: For immunoblotting the following primary antibodies were used: MICA/B (F-6) (1:500, sc-137242, Santa Cruz Biotechnology), ULBP2 (1:1000, ab275023, AbCam), GM130 (1:10000, 12480, Cell Signaling Technology). β-Actin (1:2000, TA811000, Origene), was used as loading control for total cell lysates and cytoplasmic fraction, while TATA-binding protein (TBP, 1:1000, ab51841, AbCam), and Na + /K + -ATPase (1:20000, ab76020, AbCam) were used as loading controls for nuclear and membrane fractions, respectively.

    Techniques: Staining, Western Blot, Membrane, Binding Assay